Status and Need of Research on Rhizobia and Arbuscular Mycorrhizal Fungi Associated with Leguminous Plants in Saudi Arabia

Mosbah Mahdhi, Taieb Tounekti, Habib Khemira


Most regions of Saudi Arabia are frequently subjected to high temperature and drought spells which destroy natural vegetation and lead to soil erosion and the advance of sand dunes.  The use of legume plants may help to preserve these ecosystems and to slow down desertification processes. Legumes produce extensive, deep root systems with a capacity to develop mycorrhizal and rhizobial symbioses facilitating the plant’s nutrition via enhanced soil nutrients absorption and atmospheric nitrogen fixation, respectively. These associations play a vital role in preserving and even restoring the fertility of poor and eroded soils. Research on tripartite associations of Arbuscular mycorrhizal fungi (AMF), rhizobia and legumes is rare in Saudi Arabia. Little is known about the diversity of these beneficial symbioses in Saudi ecosystems and their beneficial role for the sustainable management of range land. We attempted to explore the present status of research on rhizobia and AMF associated with various legumes in Saudi Arabia. The use of legume plants associated with microbial symbionts may be a useful asset in preserving fragile ecosystems and combating desertification. The identification of adapted and efficient legume-rhizobia-fungi tripartite associations in local ecosystems is of paramount importance for successful ecosystem restoration, rangeland and forest management and agricultural sustainability especially under unfavorable environmental conditions due to soil salinity, drought and heat stress.


rhizobia; Arbuscular mycorrhizal fungi; legumes; Saudi Arabia


Gamalero E, Glick BR. “Mechanisms used by plant growth-promoting bacteria. In: Maheshwari DK, ed. Bacteria in Agrobiology: Plant Nutrient Management. Heidelberg: Springer; 17-46. 2011

Graham PH, and Vance CP. Legumes: importance and constraints to greater use. Plant Physiol. 2003, 131:872-877

Morris JB. Special-purpose legume genetic resources conserved for agricultural, industrial, and pharmaceutical use. Economic Botany. 1997, 51:251-263

Kennedy AR. The evidence for soybean products as cancer preventive agents. J Nutr. 1995, 125: 733-743

Mahdhi M, and Mars M. Genotypic diversity of rhizobia isolated from Retama raetam in arid regions of Tunisia. Ann Microbiol. 2006, 56 (4):305-311

Mahdhi M, Nzoué A, Gueye F, Merabet C, de Lajudie P, and Mars M. Phenotypic and genotypic diversity of Genista saharae microsymbionts from the infra-arid region of Tunisia. Lett Appl Microbiol. 2007, 54:604-609

Mahdhi M, de Lajudie P, and Mars M. Phylogenetic and symbiotic characterization of rhizobial bacteria nodulating Argyrolobium uniflorum in Tunisian arid soils. Can J Microbiol. 2008, 54 (3):209-217

Zahran HH. Structure of root nodules and nitrogen fixation in Egyptian wild herb legumes. Biol Plantarum. 1998, 41: 575-585

Alfarhan AH, Al-Turki TA, Thomas J, and Basahy RA. Annotated list to the flora of Farsan Archipelago, Southern Red Sea, Saudi Arabia. Taeckholmia. 2001, 22(2):1-33

De Pauw E. An agroecological exploration of the Arabian Peninsula. Syria: ICARDA, Aleppo, 2002

Fisher M, and Membery DA. Vegetation of the Arabian Peninsula. In: Fisher M, Membery DA, eds. Geobotany: vegetation of the Arabian Peninsula. Dordrecht: Kluwer Academic Publishers; 1998: 5-38

Aldhebiani AY, and Howladar SM. Floristic Diversity and Environmental Relations in Two Valleys, South West Saudi Arabia. Int J Sci Res. 2015, 4 (2):1916-1925

Hussain G, Alquwaizany A, and Al-Zarah A. Guidelines for Irrigation Water Quality and Water Management in The Kingdom of Saudi Arabia: An Overview. J Appl Sci. 2010, 10:79-96

Masrahi YS, Al-Turki TA, Sayed OH. Wolffiella hyalina (Delile) Monod (Lemnaceae) – a new record for the flora of Saudi Arabia. Feddes Repertorium. 2010, 121:189-193

Chaudhary SA. Destructions of Acacia woodlands and Juniper forests in Asia and Eastern Africa. Pakistan J Bot. 2010, 42, 259-266

Collenette S. Wild Flowers of Saudi Arabia. Saudi Arabia: National commission for Wildlife Conservation and Development; 1999

Almohisen IA, and Alyemeni MN. Changes in the yield quantity and quality of the pods of four legume crops in response to elevated ambient ozone in Riyadh, South Asian J Exp Biol. 2013, 3:97-105

Abdel-Aziz RA, Al-Barakah FN, and Al-Asmary HM. Genetic identification and symbiotic efficiency of Sinorhizobium meliloti indigenous to Saudi Arabian soils. Afr J Biotechnol. 2008, 7 (16), 2803-2809

Al-Fredan MAA. Nitrogen Fixing Legumes in the Plant Communities. American J Envir Sci. 2011, 7 (2):166-172

Thomas J, Basahi R, Al-Ansari AE, Sivadasan M, El-Sheikh MA, Alfarhan AH, and Al-Atar AA. Additions to the Flora of Saudi Arabia: Two New Generic Records from the Southern Tihama of Saudi Arabia. National Academy Science Letters India 2015, 38(6):513-516

Wickens GE, Seif El Din AG, Sita G, and Nahal I. Role of Acacia species in the rural economy of dry Africa and the Near East. Rome: FAO Conservation Guide No. 27; 1995

Fox JED. A review of the ecological characteristics of Acacia saligna (Labill.) H. Wendl. Mulga Research Centre Journal. 1995, 12:39-56

Shetta ND, Alshahrani TS, and Abdel-Aal MA. Identification and Characterization of Rhizobium Associated with Woody Legume Trees Grown under Saudi Arabia Condition. Am Eurasian J Agric Environ Sci. 2011, 10 (3):410-418

Shetta D, EL-Sayed AWB, Nasr TA, and Shaarawy NM. Influences of mineral fertilization with NPK, inoculation and methods of inoculation on seedling growth of two woody legume trees. World Appl Sci J. 2014, 29 (7):825-834

Alshaharani TS, and Shetta ND. Phenotypic and biochemical characterization of root nodule bacteria naturally associated with woody tree legumes in Saudi Arabia. J Envir Biol. 2015, 36 (2):363-370

Shetta ND, and Alshahrani TS. The Symbiotic Efficiency of Legume Tree Rhizobia for Host Range Legumes in Central Saudi Arabia. Int J Agric Biol. 2016, 18 (4):851-857

Mashhady AS, Salem SH, Barakah FN, and Heggo AM. Effect of salinity on survival and symbiotic performance between Rhizobium meliloti and medicago sativa l. in Saudi Arabian soils. Arid Soil Res Rehabil. 1998, 12:3-14

Khalifa AYZ, and Almalki MA. Isolation and characterization of an endophytic bacterium, Bacillus megaterium BMN1, associated with root-nodules of Medicago sativa L. growing in Al-Ahsaa region, Saudi Arabia. Ann Microbiol. 2015, 65(2):1017-1026

AL-Ghamdi AAM, Jais HM, and Khogali A. Relationship between the status of arbuscular mycorrhizal colonization in the roots and heavy metal and flavonoid contents in the leaves of Juniperus procera. J Ecol Nat Environ. 2012, 4:212-218

AL-Ghamdi AAM, and Jais HM. Interaction between Arbuscular Mycorrhiza and Heavy Metals in the Rhizosphere and Roots of Juniperus procera. Int J Agric Biol. 2012, 14:69-74

Al-Khaliel AS. Effect of salinity stress on mycorrhizal association and growth response of peanut infected by Glomus mosseae. Plant Soil Environ. 2010, 56:318-324

Al-Qarawi AA, and Alshahrani TS. Growth Response of Two Species of Zizyphus to Inoculation with Arbuscular Mycorrhizal Fungi. Met Env Arid Land Agric. Sci. 2010, 21:109-122

Abdel-Fattah GM, and Asrar AWA. Arbuscular mycorrhizal fungal application to improve growth and tolerance of wheat (Triticum aestivum L.) plants grown in saline soil. Acta Physiol Plant. 2012, 34:267-277

Al-Qarawi A, Mridha MAU, Dhar PP, and Alghamdi OM. Management Of Arbuscular Mycorrhizal Fungi By Growing Petunia Hybrida (L.) Mill. As An Ornamental Plant In Saudi Arabia - A Case Study. Pakistan J Bot. 2014, 46:749-752

Dreyfus BL, and Dommergues YR. Nodulation of Acacia species by fast- and slow-growing tropical strains of Rhizobium. Appl Envir Microbiol. 1981, 41(1):97-99

36 Van Der Heijden MGA, Klironomos JN, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A, and Sanders IR. Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature. 1998, 396:72-75

Van Der Heijden MGA, Bakker R, Verwaal J, Scheublin TR, Rutten MV, Logtestijn RSP, and Staehelin C. Symbiotic bacteria as a determinant of plant community structure and plant productivity in dune grassland. FEMS Microbiol Ecol. 2006, 56:178-187

Chalk PM, Souza RDF, Urquiaga S, Alves BJR and Boddy RM. The role of arbuscular mycorrhiza in legume symbiotic performance. Soil Biol Biochem. 2006, 38:2944-2951

Abd-Alla MH, El-Enany A-WE, Nafady NA, Khalaf DM, and Morsy FM. Synergistic interaction of Rhizobium leguminosarum bv. viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soil. Microbiol Res. 2014, 169 (1):49-58

Porcel R, Aroca R, and Ruiz-Lozano JM. Salinity stress alleviation using arbuscular mycorrhizal fungi. A review. Agron Sustain Dev. 2012, 32:181-200

Ding X, Zhang S, Wang R, Li S, and Liao X. AM fungi and rhizobium regulate nodule growth, phosphorous (P) uptake, and soluble sugar concentration of soybeans experiencing P deficiency. J Plant Nutr. 2016, 39 (13):1915-1925

Tahira Y, Sohail H, Mohsin T, and Shafaqat A. Significance of arbuscular mycorrhizal and bacterial symbionts in a tripartite association with Vigna radiate. Acta Physiol Plant. 2012, 34(4):1519-1528

Mugabo JP, Balkrishna SB, Anil K, Havugimana ., Byiringiro E, and Yumnam NS. Contribution of Arbuscular Mycorrhizal Fungi (AM Fungi) and Rhizobium Inoculation on Crop Growth and Chemical Properties of Rhizospheric Soils in High Plants. J Agric Vet Sci. 2014, 7 (9):45-55

Van der Heijden MGA, de Bruin S, Luckerhoff LV, Logtestijn RSP, and Schlaeppi K. A widespread plant-fungal-bacterial symbiosis promotes plant biodiversity, plant nutrition and seedling recruitment. The ISME Journal. 2016, 10:389-399

Nasto MK, Alvarez-Clare S, Lekberg Y, Sullivan BW, Townsend AR, and Cleveland CC. Interactions among nitrogen fixation and soil phosphorus acquisition strategies in lowland tropical rain forests. Ecol Lett. 2014, 17:1282-1289

Scheublin TR, Ridgway KP, Young JPW, and Van der Heijden MGA. Nonlegumes, Legumes, and Root Nodules Harbor Different Arbuscular Mycorrhizal Fungal Communities. Appl Envir Microbiol. 2004, 70 (10):6240-6246

Antunes PM, de Varennes A, Rajcan I, and Goss MJ. Accumulation of specific flavonoids in soybean (Glycine max (L.) Merr.) as a function of the early tripartite symbiosis with arbuscular mycorrhizal fungi and Bradyrhizobium japonicum (Kirchner) Jordan. Soil Biol Biochem. 2006, 38:1234-1242

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