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Antifungal activity of mangrove rhizobacterium Pseudomonas aeruginosa against certain phytopathogenic fungi and its growth characterization

Suppaiah Sankaralingam, S. Palpperumal, D. Kathiresan, B. Harinathan, T. Shankar, D. Prabhu

Abstract


Antimicrobial substances are widespread and they are likely to play an important protective role. Marine bacterium has been recognized as producer of important antimicrobial substances which has an exceedingly bright future in the discovery of life saving drugs. The present study was carried out to screen the antifungal activity of mangrove rhizobacteria against certain phyto pathogens from Manakudi estuary, Kanyakumari District, Tamilnadu. Around 20 colonies obtained in Zobell marine agar plates were screened for antifungal traits. Among the 20 isolates, the candidate bacterial isolate exhibited good anti fungal ability. Identification of strains was carried out and confirmed by cultural, biochemical and 16S rDNA sequences. The potent strain was identified as Pseudomonas aeruginosa. Various process factors such as different pH, temperature, carbon and nitrogen sources and NaCl were tested for the bacterial growth in static and shaking conditions. The isolated Pseudomonas aeruginosa possesed a variety of promising properties that favoured as a better biocontrol agent. In the present investigation antifungal activity of the mangrove isolate was tested against common pathogens like Penicillium sp., Candida sp., Aspergillus sp., Aspergillus fumigatus, Aspergillus flavus, Pescalotionbsis sp., Fusarium oxysporum and Glomerella cinculata. The candidate bacterium showed inhibitory action to the tested fungal pathogens except Fusarium oxysporum and Glomerella cinculata.

 


Keywords


Pseudomonas aeruginosa; fungal pathogens; antifungal activity; Mangrove Rhizobacteria

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