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Re-evaluation of Metaplastic Osteogenesis in the Bovine Lymph Node with Metastatic Cholangiocarcinoma: A Supplemental Report

Susumu Ohfuji


Introduction: For deepening knowledge of the developmental process involving metaplastic osteogenesis in extraskeletal tumor, histopathological re-evaluation was conducted on osseous metaplasia in the bovine lymph node with metastatic cholangiocarcinoma, which was previously reported in this journal by the author.

Presentation of Case: In an aged Holstein-Friesian dairy cow that exhibited intrahepatic cholangiocarcinoma with widespread metastasis, a mesenteric lymph node with metastatic cancer had multifocal areas of osseous metaplasia. Abundant stromal connective tissue of the metastatic cancer showed prominent proliferation of capillaries and mesenchymal cells. There were no definite transitional features between mesenchymal cells associated with bone tissue and cancer cells. A variety of stages toward differentiating into osseous component included occurrence of (1) focal areas of dense collagen fibers accompanied by many mesenchymal cells, (2) osteoid surrounded by a single to multilayer of osteoblasts, (3) trabeculae of woven bone containing many osteocytes, and (4) bone resorption associated with osteoclastic activity in more differentiated bone trabeculae. These findings suggested that osseous metaplasia might have developed through the process identical to intramembranous ossification, as seen in flat bone formation in the developing fetus. Furthermore, this metaplastic osteogenesis was associated with the process involving the coordinated activities of osteoblasts and osteoclasts, as seen in remodeling of bone tissue throughout postnatal life.

Conclusion: Although metaplastic bone did not fully differentiated into mature bone, the pathway of metaplastic osteogenesis might have followed the developmental stages identical, to some degree, to the process that controls skeletal maturation and remodeling.



Bovine; lymph node; metaplastic osteogenesis; metastatic cancer


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