Cover Image

Cytogenetics and FISH Studies in Multiple Myeloma – A Retrospective Study from Western India

Pankaj K. Gadhia, Salil Vaniawala

Abstract


Multiple myeloma is characterized by a complex pattern of extensive genomic aberrations involving many chromosomes and it constitutes about 1% of all malignancies. We have performed conventional cytogenetic (CC) and interphase FISH on 58 cases of MM. Results showed that from 58 cases, only 08 cases had abnormal karyotype by conventional cytogenetic. On the other hand, interphase FISH study with 58 MM patients revealed 08 patients with normal results while 50 patients showed complex genetic aberrations. It included deletions of 13q14 (48.3%), 17p13 (13.8%), 11q13 (27.6%) along with translocation of IgH involving t(4;14)(51.7%) and t(14;16)(1,7%). We conclude that interphase FISH study should be performed in conjunction with conventional cytogenetic for prognostic significance in MM.

Keywords


Multiple myeloma; Bone marrow; G-banding, Karyotyping; FISH

References


Tricic RL, Skelin IK, Sustercic D, Planinc-Periaca A, Ajdukovic R, Harris V, et al. Cytogenetic of multiple myeloma. Coll Anthropol. 2010, 34: 41-44

Ghalaut PS, Chaudhri S, and Singh R. Recent advances in diagnosis and management of multiple myeloma. 2008, chapter 80, section 10, 360-365

Kapoor P, Fonseca R, Rajkumar V, Sina S, Gretz MA, Stewert K, Bergsagel PL, Lacy MQ, et al. Evidence of cytogenetic and FISH risk stratification of newly diagnosed MM in the era of novel therapies. Myo Clin Proc. 2010, 6:532-537

Hallek M, Bergsagel PL, and Anderson KC. Multiple myeloma: increasing evidence for a multistep transformation protest. Blood. 1998, 91:3-21

Usmani SZ, Crowley J, Hoering A, Waheed S, et al. Improvement in long-term outcomes with successive total therapy trials for multiple myeloma: Are patients now being cured? Leukemia. 2013, 27:226-232

Nadiminti K, Fenhaung Z, and Guido T. Cytogenetics and chromosomal abnormalities in multiple myeloma – A review. Cloning and Transgenesis. 2013, 2(3):1-10

Toon G, Molecular pathogenesis of multiple myeloma. Hematol Oncol Clin N Am. 2007, 21:985-1006

Mohamed AN, Bentely G, Bonnett ML, Zonder J, Al-Katib A. Chromosome aberrations in a series of 120 multiple myeloma cases with abnormal karyotypes. Am J Hematol. 2007, 82(12):1080-1087

Seabright M. A rapid banding technique for human chromosome. Lancet. 1971, 2:971-972

International System for Human Cytogenetic Nomenclature (ISCN). S. Kargar Pub. Inc. 2009

Saraf S, Patel P, and Rondoll D. Epidemiology, Biology and outcome in multiple myeloma patients in different geographical areas of the world. J Ad Int Med. 2012, 1(1):20-32

Kurahashi S, Sawamoto A, Sugianoto T, Narimatsu H, Iwaski T, Adachi T, et al. Frequency and prognostic value of chromosome abnormalities in multiple myeloma. Rinsho Ketuki. 2007, 48:1455-1461

Bang SM, Kim YR, Cho HI, Chi HS, Seo EJ, Park CJ et al. Identification of 13q deletion, trisomy 19 and IgH rearrangement as the most frequent chromosomal changes found in Korean patients with multiple myeloma. Can Genet Cytogenet. 2006, 168:124-132

Tiong LAS, Hui LT, Shien SKH, Jun NU, Min TY, Lian CNS, Xiner LS, Yenny T, et al. Cytogenetic and molecular aberrations of multiple myeloma patients: a single centre study in Singapore. Chin Med J. 2013, 126(10):1872-1877

Sawyer JR, Luckacs JL, Thomas EL, Swanson CM, Goosen LS, Sammaritino G, et al. Multicolour spectral karyotyping identifies new translocations and a recurring pathway for chromosome loss in multiple myeloma. Br J Hematol. 2001, 112:167-174

Gonzalez MB, Hernadez JM, Gracia JL, Lumbreras E, Castellanos M, Hernandez JM et al. The value of fluorescence in situ hybridization for the detection of 11q in multiple myeloma. Hematologica. 2004, 89(10), 1213-1218


Full Text: PDF

Refbacks

  • There are currently no refbacks.


AJCurrB Copyright © 2012-2018. All rights reserved. Published by Ivy Union Publishing, 3204 Valley Rush Dr, Apex, North Carolina 27502, United States