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Evaluation of Antibacterial Activity and Cytotoxicity of Green Synthesized Silver Nanoparticles Using Hemidesmus Indicus R.Br.

Kalyani Khanra, Anupam Roy, Nandan Bhattacharyya

Abstract


In this work, Silver nanoparticle with potent antimicrobial activity was produced by plant mediated green synthesis of silver nitrate. Characterization of silver nanoparticle revealed that nanoparticles were crystalline in nature. TEM analysis confirmed the spherical shape of nanoparticle billow 20 nm of size. X-ray diffraction analysis reveals that the silver nanoparticles are crystals. Investigation of the antibacterial properties of silver nanoparticles against gram positive S. aureus and gram negative P. aeruginosa offered a minimum inhibition concentration of 12.5 µg/ml for both the cases. In addition, silver nanoparticles were able to decrease the viability of both PA-1 cell line (derived from human ovary Teratocarcinoma), and A549 (human non-small cell lung alveolar epithelial cell line) in a dose dependent manner.


Keywords


Green synthesis; silver-nanoparticle; Antimicrobial; cytotoxic

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