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Philip Ogbeide Orhue, Faith Oseahumen Okoebor, McSionel Abdul-Razaq Momoh


This study investigates the effect of plasmid curing on antibiotics susceptibility pattern of Pseudomonas aeruginosa clinical isolates. The study involved confirmed clinical samples of P. aeruginosa obtained from patients attending the general out-patient department of Irrua Specialist Teaching Hospital, Irrua-Nigeria. Samples with established Pseudomonas aeruginosa were subjected to antibiotic susceptibility testing before plasmid curing (group 1) and after plasmid curing (group 2) using Sodium Dodecyl Sulfate intercalating dye following previously determined standard procedures. Antibiotic resistivity and susceptibility outcomes were then determined by measuring the zone of inhibition and the pre and post cured experiments were compared. The results showed that Pseudomonas aeruginosa is 100% resistance to tetracycline, cefotaxime, ethromycin, methronidazole, nitrofuratoin, ampicillin-clauvulanate, ampicillin, amoxicillin and ampiclox. Post-plasmid curing resulted to increased susceptibility rangeing from 13.3% to 92.0% as against 5.3% to 80% in the pre-plasmid cured P. aeruginosa. Based on these findings, clinical isolates P. aeruginosa in the study area has a very high resistance rate that may be due to plasmid development or mutation of the organism and thus, the call for clinicians to carryout antibiotics susceptibility testing prior to antibiotic prescription.


Pseudomonas aeruginosa, antibiotics susceptibility, plasmid curing, clinical isolates

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