Epidemiological Study on Rift Valley Fever Virus among Domestic Animals in Taiz Governorate (Yemen)

Mansour Shayif Al-aesayi, Mohammed F. Al-Helali, Jamil S. Mubarak, Saeed M. Al-Galibi, Ahmed M. Al-Qadasi


Rift Valley Fever Virus (RVFV) is the causative agent of Rift Valley Fever (RVF), transmitted either by hematophagous insects that infect animals and humans or by direct contact with infected animals. This study was performed to preparing RVF antigen from liver of infected lamb to be used in detecting anti-RVFV antibodies by using Enzyme Linked immunosorbent assay (ELISA) and to study epidemiological prevalence of anti-RVFV IgM and IgG antibodies among animals which conducted in the Researches Center of Taiz University and Central Health Laboratory, Taiz Governorate, Southwestern Yemen. RVFV antigen was successfully prepared at a titer dilution of 1: 3200 by Chessboard (CB) ELISA assay. However, out of 188 samples, 147 samples were positive for ELISA assay, of which 12 (8.2 %) were positive for anti-RVFV IgM and 135 (91.8 %) for anti-IgG antibodies. The antigen was successfully produced, and successfully adhered to ELISA plates. Simple and inexpensive methods give good results. The results can be used to develop and refine predictive database for RVF transmission based on environmental and remote sensing data.


Rift Valley fever; antigen; ELISA; Epidemiology; Yemen

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Sasita Shabani S, Mangi JE, Mohamed M and Candida SM. Knowledge, attitudes and practices on Rift Valley fever among agro pastoral communities in Kongwa and Kilombero districts, Tanzania. BMC Infectious Diseases. 2015, 15 (1): 356

Laughlin LW, Meegan JM, Strausbaugh LW, Morens DM and Watten RH. Epidemic Rift Valley fever in Egypt, observation of the spectrum of human illness. Trans R Soc Trop Med Hyg. 1979, 73(6):630-633

Office International des Epizooties/World organization for Animal Health. Rift valley fever., Jowa State University, College of veterinary Medical, RVF_H1106_0507. OIE, 2007

Tariq A, Madani YY, Al-Mazrou MH, Al-Jeffri AA, Mishkhas AM, et al. Rift Valley Fever Epidemic in Saudi Arabia: Epidemiological, Clinical, and Laboratory Characteristics. Clinical Infectious Diseases, 2003, 37(8):1084-1092

Elizabeth Cook, Grossi-Soystter EN, de Glanville WA, Thomas LF, Kariuki S, et al. The sero-epidemiology of Rift Valley Fever in people in the lake Victoria basin of western kenya. PLoS Negl Trop Dis. 2017, 11(7): e0005731

Pepin M, Bouloy M, Brian HB, Kemp A and Janusz Paweska J. Rift valley fever virus (Bunyaviridae: Phlebovirus): an update on pathogenesis, molecular epidemiology, vectors, diagnostics and prevention. Vet Res. 2010, 41 (6):125-128

Chaniotis B. Arthropod-borne viral zoonoses in the Mediterranean area (An overview). Information circular-WHO Mediterranean Zoonoses Control Center, 2003, 56:1-16

Seleem MN, Asmaa Hussein, Ismail AA and Nafie EK. Serological studies on RVF in some patients of fever hospital in Upper Egypt. J Egypt Vet Med Assoc. 2002, 62(6a):261-271

World Health Organization. Rift Valley fever outbreak., Disease Outbreak Reported, Global Alert and Response (GAR), 2000

Abdo-Salem AS, Waret-Szkuta A, Roger F, Olive MM, Saeed K and Chevalier V. Rift valley fever in yemen. Trop Anim Health Prod. 2011, 43 (2):471-480

Boushab MB, Fatima Zahra, Sidi WOB, Mohamed LOS, Marie Roseline DB, et al. Sever human illness caused by Rift Valley Fever Virus in Mauritania. Oxford University Press. Open Forum Infect Dis. 2016, 3 (4):ofw200

Scott RM, Feinsod FM, Allam IH, Ksiazek TG, Peters CJ, et aL. Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta. J Clin Microbiol. 1986, 24(4): 612-614

Burleson FG, Chambers TM and Wiedbrauk DL. Virology: a laboratory manual. 2nd edition, Academic Press San Diego, Sydney, 1992

Provenzano M, Rossi CR, Mocelline S. The usefulness of quantitative Real time PCR in immunogenetics. ASHI quarterly, Third quarter 2001

Smithburn KC, Haddow AJ and Lumsden WHR. Rift Valley Fever; Transmission of the virus by mosquitoes. Br J Exp Pathol. 1948, 30 (1):35-47

Clarke DH and Casals J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958, 7(5):561-573

Rose NR, Friedman H and Fahey JL. Manual clinical laboratory immunology, 3rd edition. Am Soc For Microbiology, Washington, DC, 1986, 107-109

Voller A, Bidwell D and Bartlett A. Microplate enzyme immunoassays for the immunodiagnosis of virus infection, Am Soc for Microbiology. 1976, 506-512

Said, Taradi Abd El fatah. Production of some diagnostic reagents for Rift Valley fever. PhD thesis, Cairo University, Faculty of Vet. Medicine, Department of Virology, 2003.

Paweska JT, Barnard BJ and Williams R. The use of sucrose acetone extracted RVFV antigen derived from cell culture in an indirect Elisa and HI. Onderstepoort, J Vet Res., Dec., 1995, 62(4): 227-233.

Maysa Awadallah. Some epidemiological studies on Rift Valley Fever. PhD thesis, Zagazig Uni., Faculty of Vet. Med. Zoonoses

Thonnon J, Picquet M, Thiongane Y, Mustapha LO, Sylla R, et al. RVF surveillence in the lower Senegal River basin: update 10 years after the epidemic. Trop Med International Health. 1999, 4(8):580-585

Zeller HG, Akapko AJ and Ba MM. RVF epizootic in small ruminants in Southern Mauritania (October 1993): risk of extensive outbreaks. Ann Soc Belg Med Trop., 1995 June., 75(2): 135-140.

Nabeth P, Kane Y, Abdalahi Mo, Diallo M, Ndiaye K, et al. Rift Valley Fever outbreak, Mauritania, 1998: Seroepidemiologica, Virologica, Entomologic and Zoologic investigation. Emerg.Infect.Dis. 2001, 7 (6):1052-1054

Pepin M, Paweska J and Bouloy M. Diagnostic specificity of ELISA-based tests for the detection of antibodies to Rift Valley Fever virus in French ruminants. Revue Méd Vét. 2010, 161(3):104-107

Joshi MV, Elankumaran S, Joshi GD, Albert A, Padbidri VS, et al. A post-epizootic survey of RVF-like illness among sheep at Veerapuram, Chennai, Tamil Nadu. Indian J of Virology, 1998, 14(2): 1-15; 10 ref.

Youssef AI. An epidemiological studies on RVF in Ismailia province. M.V.Sc. thesis (master degree), Department of Animal Hygiene, Behaviour and Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Egypt, 2004

Byomi AM, Samaha HA, Zidan, SA and Hadad GA. Some associated risk factors with the ocurrence of Rift Valley fever in animals and man in certain localities of Nile Delta, Egypt. Assiut Vet Med J. 2015;16 (61):144

Qlaleye OD, Tomori O, Ladipo MA and Schmitz H. Rift Valley fever in Nigeria: infection in humans. Rev Sci Tech. 1996, 15(3):923-935

Yedloutschnig RJ, Dardiri AH and Walker JS. Persistence of RVFV in spleen, liver, and brain of sheep after experimental infection. Contrib Epidemiol Biostat. 1981, 3:2-7


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