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Cold-active Moulds from Jammu and Kashmir, India as Potential Source of Cold-active Enzymes

Sanjay Sahay, Mansoor A Lone, Preeti Jain, Poonam Singh, Deepak Chouhan, Fakhar Shezad

Abstract


Cold-active moulds have been isolated from the soil of ten selected sites of Jammu and Kashmir (India) in the winter season. Most of them turned out to be psychrotolerant except BPF-5 and BPF-6 which showed defective growth above 20oC, and thus were identified as psychrophilic moulds. BPF-5 was also found to form sexual structure at 4oC, while BPF-6 formed melanaceous filaments in old culture. The isolate BPF-5 has been identified as Truncatella angustata and BPF-6 as Psudogymnoascus sp. Among psychrotolerant moulds, the species of Cladosporium and Penicillium were found to be dominant taxa in terms of frequency and number of species while Rhizomucor sp., to be the most prolific mould under in vitro culture. Many of them formed adaptive structures and pigment.  All of these isolates were able to utilize starch, cellulose, casein and tween-80 while many of them were able to use pectin and carboxy methyl cellulose (CMC) as sole carbon source at 4oC suggesting that they might be important sources of cold-adapted enzymes and other biomolecules. Although α-amylase from all the isolates showed residual cold-activity, that from BPF-6 exhibited the highest one suggesting it to be further explored for biotechnological applications.


Keywords


Cold-adapted moulds; Baramulla-soil; Psychrophilic moulds; Psychrotolerant moulds

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El-Meleigy MA, Hoseiny EN, Ahmed SA, Al-Hoseiny AM.. Isolation, identification, morphogenesis and ultrastructure of obligate halophilic fungi. J Appl Sci Environ Sanit. 2010, 5:201-212

Morita RY. Psychrophilic Bacteria. Bacteriol Rev. 1975, 39: 144-167

Russell N. Culture (Oxoid). 2006, 27:1-4

Robinson CH. Cold adaptation in Artic and Antarctic fungi. New Phytology. 2001, 151:341

Singh SM, Puja G, Bhat DJ. Psychrophilic fungi from Schirmacher oasis, East Antarctica. Curr sci. 2006, 90:1388-1392

Gounot A-M. Bacterial life at low temperature:physiological aspects and biotechnological implications. J Appl Bacteriol. 1991, 71:386-397

Hoover RB, Pikuta EV, Townsend A, Anthony J, Guisler M, McDaniel, Bej A, Storrie-Lombardi M. Microbial extremophiles from the 2008 Schirmacher Oasis Expedition:preliminary results. Proceeding of SPIE 7097, 2008, 70970L

Feller G, Gerday C. Psychrophilic enzymes: molecular basis of cold adaptation. CMLS, 1997, 53:830-841

Sahay S, Hamid B, Singh P, Ranjan K, Chauhan D, Rana RS, Chaurse VK. Evaluation of pectinolytic activities for oenological uses from psychrotrophic yeasts, Lett Appl Microbiol. 2013, 57:115-121

Singh P, Hamid B, Lone MA, Ranjan K, Khan A, Chaurse VK, Sahay S. Evaluation of pectinase activity from the psychrophilic fungal strain Truncatella angustata - BPF5 for use in wine industry. Journal of Endocytobiosis and Cell Research. 2012, 22:57-61

Del Frate G, Caretta G. Fungi isolated from Antartic material. Polar biol. 1990, 11:1-7

12. Möller C, Dreyfuss MM.Microfungi from Antarctic lichens, mosses and vascular plants. Mycologia. 1996, 88, 922-933

Kumar PS, Ghosh M, Pulicherla KK, Rao KRSS. Cold Active Enzymes from the Marine Psychrophiles: Biotechnological Perspective. Advanced Biotechnol, 2011, 10:16-20

Weinstein RN, Montiel PO, Johnstone K. Influence of growth temperature on lipid and soluble carbohydrate synthesis by fungi isolated from fell field soil in the maritime Antarctic. Mycologia. 2000, 92:222-229

Gaudet DA, Laroche A, Yoshida M. Low temperature-wheat-fungal interactions: a carbohydrate connection. Physiologia Plantarum. 1999, 106:443

Snider CS, Hsiang T, Zhao G, Griffith M. Role of ice nucleation and antifreeze activities in pathogenesis and growth of snow moulds. Phytopathol. 2000, 90:354-361

Tibbett M, Sanders FE, Cairney JWG. The effect of temperature and inorganic phosphorus supply on growth and acid phosphatase production in arctic and temperate strains of ectomycorrhizal Hebelomaspp. in axenic culture. Mycol Res. 1998, 102:129-135

Rothschild LJ, Mancinelli RL. Life in extreme environments, Nature. 2001,409: 1092-1101

Flanagan PW, Scarborough AM. Physiological groups of decomposer fungi on tundra plant remains. In: Holding AJ, Heal OW, MacLean Jr SF, Flanagen ed Soil organisms and decomposition in tundra. Tundra Biome Steering Committee, Stockholm, Sweden; 1974:159-181

Weinstein RN, Palm ME, Johnstone K, Wynn-Williams DD. Ecological and physiological characterisation of Humicola marvinii, a new psychrophilic fungus from fellfield soils in the maritime Antarctic. Mycologia. 1997, 89:706-711

Leotta,GA, Pare, JA, Sigler L, Montalti D, Vigo G, Petruccelli M, Enso H. The lebolus microsporus Mycelial Mats in the Trachea of Wild Brown Skua (Catharacta antarctica lonnbergi) and South Polar Skua (C. maccormicki) Carcasses Reinoso, J Wildlife Dis. 2002, 38: 443-447

Chang SW, Scheef E, Abler RA, Thomson S, Johnason P, Jung G. Distribution of Typhula spp. and Typhula ishikariensis Varieties in Wisconsin, Utah, Michigan, and Minnesota. Phytopathol. 2006, 96:926-933

Xiao N, Suzuki K, Nishimiya Y, Kondo H, Miura A, Tsuda S, Hoshino T. Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis. FEBS J. 2010, 277: 394-403

Chaturvedi V, Springer DJ, Behr MJ. Ramani R, Li X, Peck MK, Ren P, Bopp DJ, Wood B, Samsonoff WA, Butchkoski CM, Hicks AC, Stone WB, Rudd RJ, Chaturvedi S. Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS). PLoS One. 2010, 24 5:e10783

Goto S, Sugiyama J, Iizuka H. A taxonomic study of Antarctic yeasts. Mycologia.1969, 61:748-774

McRae CF, Hocking AD, Seppelt RD. Penicillium species from terrestrial habitats in the Windmill Islands, East antarctica, including a new species, Penicillium antarcticum. Polar Biol. 1999, 21:97-111

Rice AV, Currah RS. Two new species of Pseudogymnoascus with Geomyces anamorphs and theirphylogenetic relationship with Gymnostellatospora. Mycologia. 2006, 98:307-318

Espinoza JG, Briceño E X, Keith L M, Latorre B A. Canker and twig dieback of Blueberry caused by Pestalotiopsis spp. and a Truncatella sp. in Chile. Plant Dis. 2008, 92:1407-1414

Hawskwork DL. Mycologists’s Handwork: Commonwealth Mycol Inst, Kew; 1974. 30. Lowry OH, Rosebrough N, Farr A, Randall R. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951, 193:265-275

Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959, 31:426-428

32. Sagemann J, Jørgensen BB, Greef O. Temperature depen-dence and rates of sulfate reduction in cold sediments of Sval-bard, Arctic Ocean, Geomicrobiol J. 1998, 15: 85-100

Isaksen MF, Jørgensen BB. Adaptation of psychrophilic and psychrotrophic sulfate-reducing bacteria to permanently cold marine environments, App Environ Microbiol. 1996, 62:408-414

Sawicka JE, Jargensen BB, Br¨uchert V. Temperature characteristics of bacterial sulfate reduction incontinental shelf and slope sediments. Biogeosciences, 2012, 9, 3425-3435

35. Sutton, BC. The Coelomycetes: Fungi Imperfecti with Pycnidia, Acervular and Stromata; Commonwealth Mycological Institute, Kew, Surrey, England; 1980

Nakagawa T, Yamada K, Miyaji T and Tomizuka N. Cold-active Pectinolytic activity of psychrophilic- Basidiomycetous yeast Cystofilobasidium capitatum Strain PPY-1. J Biosci Bioeng. 2002, 94:175-177

37. Kostadinova N, Krumova E, Tosi S, Pashova K, Angelova M. Isolation and identification of filamentous fungi from island livingston, Antarctica. Biotechnol & Biotechnol special edition/on-line, 2009, 267-270

Burg B. 2003- Extremophiles as a source for novel enzymes. Curr Opin Microbiol. 2003, 6:213-218

Satyanarayana T, Raghukumar C, Shivaji S. Extremophilic microbes: Diversity and perspectives. Curr Sci. 2005, 89:78-90

Gerday C, Aittaleb M, Arpigny JL, Baise E, Chessa JP, Garsoux G, Petrescu I, and Feller G. Psychrophilic enzymes: a thermodynamic challenge. Biochim et Biophys Acta. 1997, 1342:119-131.

Feller G, Gerday C. 2003- Psychrophilic enzymes : hot topics in cold adaptation. Nature rev / microbiol. 2003, 1:200-208


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