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Conservational Analysis of Influenza A Virus RNA-dependent RNA Polymerase

Vivek Darapaneni

Abstract


The RNA-dependent RNA polymerase of influenza A virus is important for catalyzing the replication and transcription of viral RNA. The polymerase of Influenza A Virus plays a significant role in the infectious virus life cycle. The objective of the present study was to identify the residue conservation in polymerase complex (PA, PB1 and PB2 proteins) of Influenza A Virus. The study was based on 11,966 amino acid sequences for the PA protein; 10,605 amino acid sequences for the PB1 protein and 11,331 sequences for the PB2 protein. The PA, PB1 and PB2 proteins exhibited similar level of sequence conservation. On the whole, this study exposed residues which are universally conserved among different influenza A virus subtypes. These universally conserved residues might be involved in either structure stabilizing or protein-protein interactions. Clusters of highly variable residues detected on the surface of the PA, PB1 and PB2 proteins might be linked to adaptation to various hosts or evolutionary pressure to evade the host immune system. The conserved residues identified in the present study could form a platform for designing universal anti-influenza drugs which are resistant to mutations arising in the future.



Keywords


Polymerase; Polymerase acidic; Polymerase basic 1; Polymerase basic 2; Conservation; Mutation; Resistance; Influenza virus

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References


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