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ANTIBIOGRAM AND SUSCEPTIBILITY OF KLEBSIELLA SPP ISOLATED FROM DIFFERENT CLINICAL SPECIMENS IN HEALTH CARE CENTERS IN ETSAKO WEST LOCAL GOVERNMENT AREA OF EDO STATE, NIGERIA

Philip O Orhue, Favour R Aliu

Abstract


Arising multidrug resistance to available antibiotics is a problem of deep scientific and research concern. One organism with increasing antibiotics resistance is Klebsiella spp. that had cause several outbreaks in different locations. This study was undertaken to investigate the antibiogram and susceptibility of Klebsiella spp. isolated from clinical samples in health care centers. The study was conducted in Auchi Township in Etsako West Local Government Area, Edo State, Nigeria. Following standard procedures samples were collected and Klebsiella spp. identified using standard microbiology techniques. Antibiotic susceptibility testing was carried out using standard methods. 109 samples were collected and comprised urine (44), wound (20), high vagina swabs (17), urethral (3), sputum (9), semen (6), stool (5), endo-cervical swab (2), throat (1) and ear (1). 10 (9.17%) samples were positive to Klebsiella organism with urine consisting 50.0% and 10.0% each from sputum, stool, wound, ear and high vaginal swabs. Klebsiella isolates were completely resistance to penicillin, Amoxiclav and Chloramphenic. Amoxicillin, Floxapen, Metronidazole, Ampicillin, Cotrimoxazole, Nalidixic acid, Streptomycin, Tetracycline, Cephalexin, Genticin, Tobramycin, Nitrofurantoin, Ciprofloxacine and Cefuroxime have less than 50% potency. On the other hand, Ofloxacine, Cefotaxime, Ampisulbactam and Perflacine were 50% and above susceptible. Klesiella spp. isolated from stool sample was more multi-drug resistance (87.75%) followed by those from wound (77.55%), sputum (73.47%), high vaginal swab (69.39%), urine (65.30%) and lastly ear sample (48.98). Based on our findings, Klebsiella spp. in the study area is multidrug-resistance thus suggesting antibiotic analysis prior to treatment.


Keywords


Klebsiella Spp; Antibiogram typing; Clinical specimen; Etsako

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Brisse S, Grimont F, Grimont PAD. The genus Klebsiella. In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (editors); The Prokaryotes. A Handbook on the Biology of Bacteria, 3rd edition, Springer, New York, 2006: 159-196.

Podschun R, Ullmann U. Klebsiella spp. As nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbio. Rev., 1998; 11(4): 589-603.

Bruce, J. Automated system rapidely identifies and characterizes microorganisms in food. Food Techol., 1996, 50:77-81.

Ko WC, Paterson DL, Sagnimeni AJ, Hansen DS,Von Gottberg A, Mohapatra S, Casellas JM, Goossens H, Mulazimoglu L, Trenholme G, Klugman KP, McCormack JG,Yu VL. Community acquired Klebsiella pneumoniae bacteremia: global differences in clinical patterns Emerg. Infect Dis., 2002, 8(2):160-6.

Otman J, Perugini ME, Cavasin E, Vidotto MC. Study of an outbreak of extended-spectrum beta lactamase-producing Klebsiella species in a neonatal intensive care unit in Brazil by means of repetitive intergenic consensus sequence-based PCR (ERIC-PCR). Infect. Contr. Hosp. Epidem., 2002, 23:8-9.

Johnson AP, Weinbren MJ, Ayling-Smith B, Du Bois SK, Amyes SGB, George RC. Outbreak of infection in two hospitals caused by strains of Klebsiella pneumoniae resistant to cefotaxime and ceftazidime. J. Hosp. Infect., 1992, 20:97-103.

Arlet G, Sanson-le pors MJ, Rouveau M, Fournier G, Marie O, Schlemmer B, Philippon A. Outbreak of nosocomial infections due to Klebsiella pneumoniae producing SHV-4 beta lactamase. Eur. J. Clin. Microbiol. Infect. Dis., 1990, 9(11):797-803.

Jadhav S, Misra R, Gandham N, Ujagare M, Ghosh P, Angadi K et al; Increasing incidence of multidrug resistance Klebsiella pneumonia infections in hospital and community settings. Inter. J. Microbiol. Res., 2012; 4(6): 253-257.

Caplenas NR, Kanarek MS, Dufour AP. Source and extent of Klebsiella pneumoniae in the paper industry. Appl. Environment. Microbiol., 1981; 42(5): 779-785.

Brown C, Seidler RJ. Potential pathogens in the environment: Klebsiella pneumoniae, a taxonomic and ecological enigma. Appl. Environment. Microbiol., 1973; 25(6): 900-904.

Hansen DS, Gottschau A, Kolomos HJ. Epidemiology of Klebsiella bacteremia: a case control study using Escherichia coli bacteremia as control. J. Hospital Infect., 1998; 38(2): 119-132.

Feldman C, Smith C, Levy H, Ginsburg P, Miller SD, Koornhof HJ. Klebsiella pneumonia bacteremia at an urban general hospital. J. Infect., 1990; 20(1): 21-31.

Sikarwar AS, Batra HV. Challenge to health care: multidrug resistance in Klebsiella pneumoniae. International Proceeding of Chemical, Biological and Enviromental Engineering(IPCBEE), 2012, 9, 130-134.

Khajeh K M, et al. Antimicrobial efficacy of lytic bacteriophages against antibiotic-resistant Klebsiella species. The Scientific World J., 2011, 11, 1332-1340.

Brun-Buisson C, Legrand P, Philippon A, Montravers F, Ansquer M, Duval J. Transferable enzymatic resistance to third generation cephalosporins during nosocomial out-break of multi-resistant Klebsiella pneumoniae. Lancet, 1987, (ii):302-306.

Sirot J, Chanal C, Petit A, Sirot D, Labia R, Gerbaud G. Klebsiella pneumoniae and other enterobactericeae producing novel plasmid-mediated beta lactamases markedly active against third-generation cephalosporins: epidemiologic studies. Rev. Infect. dis., 1988, 10:867-878.

Sekowska A, Janicka G, Klyszejko C, Wojda M, Wroblewski M, Szymankiewicz M. Resistance of Klebsiella pneumoniae strains producing and not producing ESBL (extended spectrum beta lactamase) type enzymes to selected non-beta lactam antibiotics. Med. Sci. Monit., 2002, 8(3):BR100-4.

Sikarwar AS, Batra HV. Prevalence of antimicrobial drug resistance of Klebsiella pneumoniae in India. Inter. J. Biosci. Biochem. Bioinformatics, 2011, 1(3), 211-215.

Romanus II, Egwu OA, Ngozi AT, Chidiebube NA, Chika EP. Extended spectrum beta- lactamase(ESBL) mediated resistance to antibiotics among Klebsiella pneumoniae in Enugu Metropolis. Macedonian J. Med. Sci., 2009, 2(3), 196-199. 13.

Alekshun MN, Levy S.B. Molecular mechanisms of antibacterial multidrug resistance. Cell, 2007, 128, 1037–1050.

Steward CD, Wallace D, Hubert SK, Lawton R, Fridkin SK, Gaynes RP, McGowan JE, Tenover FC. Ability of laboratories to detect emerging antimicrobial resistance in nosocomial pathogens: a survey of project ICARE laboratories. Diag. Microbiol. Infect. Dis., 2000, 38 (1), 59–67.

Rupinder B, Geeta W, Shikha J. Prevalence of extended spectrum b-lactamases in multidrug resistant strains of gram negative Bacilli. J. Acad. Indus. Res., 2013, 1 (9), 558–560.

Abhijit A, Sunita N, Maria K. Study of urinary isolates with reference to extended spectrum beta lactamases detection and antibiogram. J. Evol. Med. Dent. Sci., 2013, 2 (9), 1049–1055.

Majda Q, Najma A, Summyia B. Evaluation of extended spectrumbeta-lactamase mediated resistance in Escherichia coli and Klebsiella in urinary tract infection at a tertiary care hospital. Biomedica, 2013, 29, 78–81.

Meeta S, Sati P, Preeti S. Prevalence and antibiogram of extended spectrumb-lactamase (ESBL) producing Gram negative bacilli and further molecular characterization of ESBL producing Escherichia coli and Klebsiella spp. J. Clin. Diag. Res., 2013, 7 (10), 2168– 2172.

Kritu P, Prakash G, Shiba KR, Reena KM, Ram NS. Ganesh R. Antibiogram typing of gram negative isolates in different clinical samples of a tertiary hospital. Asian J. Pharm. Clin. Res., 2013, 6 (1), 153–156.

Fatemeh A, Emran A, Elnaz K, Mohammad JGS, Mahboubeh N. The frequency of extended spectrum betalactamase (ESBL) in Escherichia coli and Klebsiella pneumonia: a report from Mashhad, Iran. J. Med. Bacteriol., 2012, 1 (3), 12–19.

Gupta V. An update on newerb-lactamases. Indian J. Med. Res., 2007, 126 (5), 417–427.

Cruickshank R, Duguid JP, Marmion BP, Swain RHA. Medical Microbiology. (12thedn). Churchill Livingstone. 1975,Vol 2. P 587.

Cowan ST, Steel JK. Manual for the Identification of Medical Bacteria. Cambridge University Press, UK. (3rd edn). 1993, P 21-171.

Chessbrough M. District Laboratory Practice Manual in Tropical Countries. Part 2. Cambridge University Press. New York. 2000, P 63-70.

Mackie M. Practical Medical Microbiology (14th Edn). Kundli press, Elsevier publishers. 2012, P 113-150.

Orhue, P.O. Antibiogram and some indigenious plant extract susceptibility profiles of Uropathogenic Bacterial isolates from University of Benin teaching Hospital, Benin City, Ph.D Thesis 2004. Ambrose Alli University, Ekpoma, Nigeria.

Joshi KR, Onaghise EO, Oyide SM, Wemumbu SNC, Uriah N. Aeruginosine typing of P. aeruginosa isolated at the University of Benin Teaching Hospital, Benin. Afr. J. Clin. Microbiol. 1984, 1 (1): 8-13.

Namratha KG, Padiyath Sreeshma, Subbannayya K, Dinesh PV, Hemachandra Champa. Characterization and Antibiogram of Klebsiella spp. Isolated from Clinical Specimen in a Rural Teaching Hospital. Sch. J. App. Med. Sci., 2015, 3(2E):878-883.

Alo MN, Saidu AY, Ugah UI, Alhassan M. Prevalence and Antbiogram of Bacterial Isolates Causing Urinary Tract Infections at Federal Teaching Hospital Abakaliki I (FETHA I). British Microbiol. Res. J., 2015, 8(2): 403-417, 2015.

Iregbu KC, Nwajiobi-Princewill PI. Urinary tract infections in a tertiary hospital in Abuja, Nigeria. Afr. J. Cln. Exper. Microbiol., 2013, 14(3): 169-173.

Abubakar EM. Antimicrobial susceptibility pattern of pathogenic bacteria causing urinary tract infections at the Specialist Hospital, Yola, Adamawa state, Nigeria. J. Clin. Med. Res., 2009, Vol. 1(1) pp. 001-008.

Romanus II, Egwu AO. Analysis of antibiotic susceptibility of Klebsiella pneumoniae isolated from different clinical specimen in Enugu State. SENRA Academic Publishers, Burnaby, British Columbia. 2011, Vol. 5, No. 3, pp. 1609-1614.

Ali Abdel Rahim KA, Ali Mohamed AM. Prevalence of extended spectrum β-lactamase producing Klebsiella pneumoniae in clinical isolates. Jundishapur J. Microbiol., 2014, 7(11): e17114.

Dalal MM, et al. Antimicrobial resistance trends of Klebsiella spp. isolated from patients in Imam Khomeini hospital. J. Faculty of Tehran University of Med. Sci. Payavard Salamat, 2012, 6(4), 275- 281.

Langarizadeh N, et al. Prevalence of multi-drug resistant (MDR) Klebsiella pneumoniae among children and adults with urinary tract infection referred to tabriz teaching hospitals. J. Biological Sci., Islamic Azad University, Zanjan, 2010, 4(1), 9-17.

Donnenberg MS. Enterobacteriaceae. In Mandel GL, Bennett JE, Dolin R editors; Mandell, Douglas and Bennett’s: Principles and practice of infectious diseases, 6th edition, Churchill Livingstone, Philadelphia, 2005, 2578-2579.

Russo TA, Johnson JR. Diseases caused by Gram negative enteric bacilli. In Fauci AS, Kasper DL, Longo DL, Braunwald E, Hausern SL, Jameson LJ et al. editors; Principles of Harrison’s Internal Medicine, 17th edition, McGraw-Hill, United States of America, 2008, 942-943.


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