Cover Image

Influence Of Anionic, Cationic And Non-Ionic Surfactants On Growth Of Hydrocarbon Utilizing Bacteria

Chikodili Gladys Anaukwu, Chinyere C. Ezemba, Vivian N. Anakwenze, Kingsley C. Agu, Amechi S. Nwankwegu, Benjamin C. Okeke, Nsikak S. Awah

Abstract


The effects of named anionic, cationic and non-ionic surfactants on growth of hydrocarbon utilizing bacteria were studied. Hydrocarbon utilizing bacteria were isolated from soil samples collected from different sampling points using mineral salt medium supplemented with hydrocarbon substrates. They were identified based on 16S rDNA sequencing. Hydrocarbons used include crude oil, kerosene, diesel and spent-engine oil. Heterotrophic bacterial count was taken to assess the bacterial count and hydrocarbon utilization of the tested hydrocarbons. Effects of Sodium dodecyl sulphate (anionic surfactant), Cetyl trimethyl ammonium bromide(cationic surfactant) and Tween-80 (non-ionic surfactant) surfactants at 0.05 - 0.30 mg/ml concentrations, on growth of the isolates were measured spectrophotometrically. Results obtained showed high bacteria count of 12x103Cfu/ml with spent oil and least (2 x 103Cfu/ml) with diesel. Five bacteria belonging to the genera Micrococcus, Serratia, Pseudomonas, Staphylococcus and Bacillus were isolated. Tween-80 stimulated the growth of all the isolates. While SDS stimulated growth of Micrococcus luteus, Staphylococcus scuiri and Bacillus cereus, it reduced the growth of  Serratia marcescens and Pseudomonas aeruginosa. CTAB was observed to improve the growth of only Pseudomonas aeruginosa. and inhibited the growth of other isolates. Statistical analysis indicates that there was significant difference on the effects of the surfactants on the growth of the isolates with p-value < 0.0001. Surface active agents increases or inhibits microbial growth at certain concentration. The use of surfactant in biostimulation of hydrocarbon degrading microorganisms is recommended. Study on the correct concentration required for growth of the microorganisms should be conducted for its efficiency and effectiveness.


Keywords


Anionic surfactant; cationic surfactant; non-ionic surfactant; microbial growth; hydrocarbon utilization

Full Text:

PDF

References


Desai JD, Banat IM. Microbial production of surfactants and their commercial potential. Microbiology and Molecular Biology Review. 1997, 61: 47- 64

Greek BF. Detergent industry ponders products for new decade. Chemical Engineeering News. 1990, 68: 25 - 52.

Aronstein BN, Alexander M. Surfactants at low concentrations stimulate biodegradation of sorbed hydrocarbons in samples of aquifer sands and soil slurries. Environ Toxicol Chem. 1992, 11: 1227-1233

Grimberg SJ, Stringfellow WT, Aitken MD. Quantifying the biodegradation of phenanthrene by Pseudomonas stutzeri P16 in the presence of a nonionic surfactant. Appl. Environ. Microbial.1996, 62:2387-2392

Okareh OT, Adebowale SA, Oyewole SA. Hydrocarbon-degrading bacteria isolation and surfactant influence on the growth of organisms: A case study in Ibadan, Nigeria. African Journal of Biotechnology. 2012, 11(63): 12657-12663

Anaukwu CG, Ekwealor AI, Ezemba CC, Anakwenze VN, Okafor UC, Archibong EJ. Pseudomonas monteilii and Citrobacter murliniae, biosurfactant-producing bacteria isolated from Nigerian soil. British Microbiology Research Journal. 2015, 10(1): 1-9

Sriparna S, Biswas D. Optimization of culture conditions for biosurfactant production from Pseudomonas aeruginosa OCD. Journal of Advanced Scientific Research.2011, 2(3):32-36

Ezemba CC, Ekwealor CC, Ekwealor IA, Ozokpo CA, Chukwujekwu CE, Anakwenze VN, Archibong EJ, Anaukwu CG. Microbacterium lacticum; a Lysine Producing Bacterium Isolated from Oil-contaminated Soil in South-East Nigeria. British Microbiology Research Journal. 2015, 9(2): 1-8

Mbachu AE, Onochie CC, Agu KC, Okafor OI, Awah NS. Hydrocarbon degrading potentials of indigenous bacteria isolated from auto-mechanic workshops at Mgbuka-Nkpor, Nigeria. Journal of Global Biosciences. 2014, 3(1):321-326. ISSN 2320-1355

Dike KS, Ekwealor IA, Eziuzor SC. Influence of antibiotics and surfactants addition on growth and methionine productivity by Bacillus cereus. Advances In Microbiology. 2013, 3: l26-131

Konieck J, Smekal F, Konickova-Radochova M. Effect of Tween 80 and dimethyl Sulfoxide on Biosynthesis of L-lysine in Regulatory Mutants of Corynebacterium glutamicum. Folia Microbiologica. 1999, 36:587-589

Ekwealor IA, Obeta JAN. Antibiotics and surfactants effects on lysine production by Bacillus megaterium. African Journal of Biotechnology. 2008, 7(10):1550-1553

Jing-Yan L, Lan-Wei Z, Ming D, Xue H, Hua-Xi Y, Chun-Feng G,Ying-Chun Z, Xue L, Yan-He Z, Ai-Ju H. Effect of Tween Series on Growth and cis-9, trans-11 Conjugated Linoleic Acid Production of Lactobacillus acidophilus F0221 in the Presence of Bile Salts. International Journal of Molecular Science.2011, 12: 9138 -9154

Van-Boxel RM, Lambrecht RS, Collins MT. Effects of polyoxyethylene sorbate compounds (Tween) on colonial morphology, growth and ultrastructure of Mycobacterium paratuberculosis. APIMS. 1990, 98: 901-908

Roy D, Minwen LM, Guangte W. Modeling of anthracene removal from soil columns by surfactant. J Environ Sci Health. 1994, 29: 197-213

Margesin R, Schinner F. Biodegradation of diesel oil by cold-adapted microorganisms in presence of sodium dodecyl sulfate. Chemosphere. 1999, 38: 3463-3472

Yalcin E, Cavusoglu K, Ozen E. Hydrocarbon degradation by a new Pseudomonas sp., strain RW-11, with polycationic surfactant to modify the cell hydrophobicity. Environmental Technology. 2011, 33: 1743-1747


Refbacks

  • There are currently no refbacks.


AJCMicrob (ISSN 2572-5815) Copyright © 2012-2020. All rights reserved. Published by Ivy Union Publishing, 3204 Valley Rush Dr, Apex, North Carolina 27502, United States