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Phytochemistry and Antimicrobial Properties of 2:1 and 1:2 Ethanol-methanol Extracts of Tetrapleura Tetraptera

Samuel Adeniyi Adeleye, OI Oguoma, Wesley Braide, Egbadon Emmanuel Osogbase


Tetrapleura tetraptera (Aidan)-an African medicinal plant was analysed for its phytochemicals and its antimicrobial activity using 1:2 and 2:1 ethanol-methanol as solvents for extraction. Amongst the phytochemicals present in the plant, oxalates, cyanogenic glycosides, tanins and phytic acids were quantified. The solvents produced different degrees of extraction of these phytochemicals as well as their antimicrobial activity. The 2:1 methanol: ethanol solvent extracted 1.425%, 0.70 mg/100g, 0.315 mg/100g, 10.805 mg/100g and 0.50% while the 2:1 ethanol: methanol solvent extracted 5.43%, 0.705 mg/100g, 0.52 mg/100g, 7.365 mg/100g and 0.635% of oxalates, cyanogenic glycosides, tanins and phytic acids respectively. Considering the antimicrobial activity of the 1:2 ethanol: methanol extracts. There was a corresponding decrease in susceptibility with decrease in the concentration of the extract indicating that the concentration of the plant extract has an effect on the isolates. The results also revealed that the isolates were all sensitive to high concentrations of 250mg/ml and 125mg/ml of the plant extract but demonstrated varied response to the 62.5mg/ml and 31.25mg/ml of the plant extract. On the other hand, the 2:1 ethanol: methanol extract was also characterized by a low activity at a low concentration of 31.25mg/ml and 62.5mg/ml. The Ethanol: methanol 2:1 extract of T. tetraptera is a better extraction method than the 1:2 ethanol: methanol extract as demonstrated by the phytochemistry and antimicrobial activity of the plant.


phytochemicals; extract; Aidan; susceptibility; antimicrobials

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