Prevalence and Characterization of Methicillin-Resistant Staphylococcus Aureus Isolates from Commonly Shared Public Objects

Nnenna Chinelo ojiagu

Abstract


The prevalence, characteristics and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on vehicular gate passes and ATMs, two most likely shared public objects, at Nnamdi Azikiwe University Awka, Anambra State, Nigeria were studied. A total of 390 of plastic gate passes and 364 of ATMs within the premises of Nnamdi Azikiwe University Awka, Anambra State, Nigeria were sampled for bacterial isolation during a period of 24 weeks. Surface swabs of samples were collected and used for S. aureus enrichment and isolation. Presumptive positive colonies were further identified Gram staining test, catalase test and coagulase test. S. aureus were screened by mecA gene amplification with multiplex PCR. Only the mecA-positive S. aureus isolates were selected for antimicrobial sensitivity assay. Antibiotic sensitivity pattern of isolates to different antibiotics was tested by disk diffusion reference method. S. aureus was isolated from 30% (117/390) of plastic gate passes and 44.8% of ATMs (163/364). With mecA amplification, 14.5% and 20.2% of the S. aureus isolates from gate passes and ATMs were identified as MRSA, respectively. Multidrug resistance to ampicillin, ceftriaxone, ciprofloxacin, erythromycin, gentamicin, oxacillin, penicillin G, rifampin, streptomycin, and tetracycline was recorded among the isolated MRSA from both sources. Implicated MRSA in samples present severe health-risk potentiality together with significant extensive antibiotic resistance.


Keywords


MRSA; ATMs; vehicular gate passes; mecA; multidrug resistance

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