Cover Image

Cellular Flow Cytometric Studies

David Aebisher, Dorota Bartusik, Jacek Tabarkiewicz

Abstract


This review focuses on flow cytometric studies at the single cell level. Currently, flow cytometry is used to analyze DNA content, cell cycle distribution, cellular viability, apoptosis, calcium flux, intracellular pH and expression of cell surface compounds in targeted cell populations. Our criteria for the selection of research papers for this review were focused on those that show current cellular applications of flow cytometry.


Keywords


Flow Cytometry; tumor; cell; cell surface compounds; targeting

Full Text:

PDF

References


Shapiro HM. The Evolution of Cytometers. Cytometry A. 2004, 58A:13-20

Maleki, MH, Devaney AJ, Schatzberg A. Tomographic reconstruction from optical scattered intensities. J Opt Soc Am. 1992, 9:1356-1363

Samra HS, He F. Advancements in High Throughput Biophysical Technologies: Applications for Characterization and Screening during Early Formulation Development of Monoclonal Antibodies. Mol. Pharmaceutics. 2012, 9:696-707

Maltsev VP. Scanning flow cytometry for individual particle analysis. Rev Sci Instrum. 2000, 71:243-255

George TC, Basiji DA, Hall BE, Lynch DH, Ortyn WE, Perry DJ, Seo MJ, Zimmerman CA, Morrissey PJ. Distinguishing modes of cell deathusing the ImageStream multispectral imaging flow cytometer. Cytometry A. 2004, 59:237-245

Galanzha EI, Kokoska MS, Shashkov EV, Kim JW, Tuchin VV, Zharov VP. In vivo fiber-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles. J Biophoton. 2009, 2:(8-9),528-539

Galanzha EI, Zharov V. Circulating Tumor Cell Detection and Capture by Photoacoustic Flow Cytometry in Vivo and ex Vivo. Cancers. 2013, 5(4):1691-1738

Alix-Panabières C, Schwarzenbach H, Pantel K. Circulating tumor cells and circulating tumor DNA. Annu Rev Med. 2012, 63:199-215

Bai H, Liu Y, Xie Y, Hoyle DL, Brodsky RA, Cheng LO, Cheng T, Wang ZZ. Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells. J Cell Physiol. 2016, 231(5):1065-1076

Moldovan A. Photo-electric technique for the counting of microscopical cells. Science. 1934, 80:188-189

Ramanathan M. Flow Cytometry Applications in Pharmacodynamics and Drug Delivery. Pharm Res. 1997, 14(9):1106-1114

Spatola BN, Murray JA, Kagnoff M, Kaukinen K, Daugherty PS. Antibody Repertoire Profiling Using Bacterial Display Identifies Reactivity Signatures of Celiac Disease. Anal Chem. 2013, 5:1215-1222

Hallam TJ, Wold E, Wahl A, Smider VV. Antibody Conjugates with Unnatural Amino Acids. Mol Pharmaceutics. 2015, 12:1848-1862

Jacobs KM, Yang LV, Ding J, Ekpenyong AE, Castellone R, Lu JQ, Hu XH. Diffraction imaging of spheres and melanoma cells with a microscope objective. J Biophoton. 2009, 2(8-9):521-527

Chang Q, Hedley D. Emerging applications of flow cytometry in solid tumor biology. Methods. 2012, 57:359-367

Ishibashi K, Tsuji A. IL-2 or IL-4 mRNA as a Potential Flow Cytometric Marker Molecule for Selective Collection of Living T Helper 1 or T Helper 2 Lymphocytes. Anal Chem. 2003, 75:2715-2723

Sack U, Tárnok A, Rothe G (eds). Cellular Diagnostics Basic Principles, Methods and Clinical Applications of Flow Cytometry. Basel, Karger, 2009, 1-28

Spyropoulos B. 50 years LASERS: in vitro diagnostics, clinical applications and perspectives. Clin Lab. 2011, 57(3-4):131-142

Quirke P, Dyson JED. Flow cytometry: Methodology and applications in pathology. J Pathol. 1986, 2:79-87

Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Nung Y, Davidson MW, Shu X. A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015, 12:763-765

Young YK, Bolt AM, Ahn R, Mann KK. Analyzing the Tumor Microenvironment by Flow Cytometry. Methods Mol Biol. 2016, 1458:95-110

Mittag A, Tarnok A. Recent advances in cytometry applications: preclinical, clinical, and cell biology. Methods Cell Biol. 2011, 103:1-20

Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK. A deep profiler’s guide to cytometry. Trends Immunol. 2012, 33(7): 323-380

Patterson JO, Swaffer M, Filby A. An Imaging Flow Cytometry-based approach to analyse the fission yeast cell cycle in fixed cells. Methods. 2015, 82:74-84

Hogg K, Thomas J, Ashford D, Cartwright J, Coldwell R, Weston DJ, Pillmoor J, Surry D, O’Toole P. Methods. 2015, 82:38-46

Telford WG, Hawley T, Subach F, Verkhusha V, Hawley RG. Flow cytometry of fluorescent proteins. Methods. 2012, 57:318-330

Kitsos CM, Bhamidipati P, Melnikova I, Cash EP, McNulty C, Furman J, Cima MJ, Levinson D. Combination of Automated High Throughput Platforms, Flow Cytometry, and Hierarchical Clustering to Detect Cell State. Cytometry A. 2007, 71A:16-27

Fattaccioli J, Baudry J, Emerard JD, Bertrand E, Goubault C, Henry CN, Bibettea J. Size and fluorescence measurements of individual droplets by flow cytometry. Soft Matter. 2009, 5:2232-2238

Morgan E, Varro R, Sepulveda H, Ember JA, Apgar J, Wilson J, Lowe L, Chen R, Shivraj L, Agadir A, Campos R, Ernst D, Gaur A. Cytometric bead array: a multiplexed assay platform with applications in various areas of biology. Clin Immunol. 2004, 110(3):252-266

Telford WG, Hawley T, Subach F, Verkhusha V, Hawley RG. Flow cytometry of fluorescent proteins. Methods. 2012, 57:318-330

Galanzha EI, Kokoska MS, Shashkov EV, Kim JW, Tuchin VV, Zharov VP. In vivo fiber-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles. J Biophoton. 2009, 2 (8-9):528-539

Siegwart AJ, Srinivasan A, Bencherif SA, Karunanidhi A, Kwon Oh J, Vaidya S, Jin R, Hollinger JO, Matyjaszewski K. Cellular Uptake of Functional Nanogels Prepared by Inverse Miniemulsion ATRP with Encapsulated Proteins, Carbohydrates, and Gold Nanoparticles. Biomacromolecules. 2009, 10(8):2300-2309

Brind’Amour J, Lansdorp PM. Analysis of repetitive DNA in chromosomes by flow cytometry. Nat. Methods 2011, 8: 484-486.

Schmidt F, Efferth T. Tumor Heterogeneity, Single-Cell Sequencing, and Drug Resistance. Pharmaceuticals (Basel). 2016, 9(2):pii: E33

Ott G, Kalla J, Hanke A, Müller JG, Rosenwald A, Katzenberger T, Kretschmar R, Kreipe H, Müller-Hermelink HK. The cytomorphological spectrum of mantle cell lymphoma is reflected by distinct biological features. Leuk. Lymphoma. 1998, 32(1-2):55-63

Ryningen A, Bruserud Ø. Epigenetic targeting in acute myeloid leukemia: use of flow cytometry in monitoring therapeutic effects. Curr Pharm Biotechnol. 2007, 8(6):401-411

Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J , Vortmeyer AO, Miller-Jensen K, Wirtz D, Fan R. High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity. Anal Chem. 2013, 85:2548-2556

Passlick B, Flieger D, Ziegler-Heitbrock HW. Identification and characterization of a novel monocyte subpopulation in human peripheral blood. Blood. 1998, 74(7):2527-2534

Zola H, Swart B, Nicholson I, Aasted B, Bensussan A, Boumsell L, Buckley B, Clark G, Drbal K, Engel P, Hart D, Horejsí V, Isacke C, Macardle P, Malavasi F, Mason D, Olive D, Saalmueller A, Schlossman SF, Schwartz-Albiez R, Simmons P, Tedder TF, Uguccioni M, Warren H. CD molecules 2005: human cell differentiation molecules. Blood. 2005, 106(9):3123-3126

Chan JKC, Ng CS, Hui PK. A simple guide to the terminology and application of leucocyte monoclonal antibodies. Histopathology. 1998, 12(5):461-480

Brackenbury R, Rutishauser U, Edelman GM. Distinct calcium-independent and calcium-dependent adhesion systems of chicken embryo cells. Proc Natl Acad Sci USA. 1981, 78(1):387-391

Brown M, Wittwer C. Flow Cytometry: Principles and Clinical Applications in Hematology. Clin Chem. 2000, 46(8):1221-1229

Kobayashi T, Yayoib E, Takatsukac Y, Maeurad Y, Furukawab J, Kitadae M, Okamotob S. Use of flow cytometry for evaluating breast cancer cell proliferation after chemotherapy. Curr Therap Res. 1995, 56(8):769-776

Joensuu H. DNA Flow Cytometry in the Prediction of Survival and Response to Radiotherapy in Head and Neck Cancer: A review. Acta Oncol. 1990, 29(4):513-516

Ishibashi K, Tsuji A. IL-2 or IL-4 mRNA as a Potential Flow Cytometric Marker Molecule for Selective Collection of Living T Helper 1 or T Helper 2 Lymphocytes. Anal Chem. 2003, 75:2715-2723

Hallam TJ, Wold E, Wahl A, Smider VV. Antibody Conjugates with Unnatural Amino Acids. Mol Pharmaceutics. 2015, 12:1848-1862

Patterson JO, Swaffer M, Filby A. An Imaging Flow Cytometry-based approach to analyse the fission yeast cell cycle in fixed cells. Methods. 2015, 82:74-84

Begg AC, Hofland I, Kummermehr J. Tumour cell repopulation during fractionated radiotherapy: correlation between flow cytometric and radiobiological data in three murine tumours. Eur J Cancer. 1991, 27(5):537-543

Jacobsen AB, Lunde S, Ous S, Melvik, Pettersen EO, Kaalhus O, Fosså SD. T2/T3 bladder carcinomas treated with definitive radiotherapy with emphasis on flow cytometric DNA ploidy values. Int J Rad Oncol Biol Phys. 1998, 17(5):923-929

Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK. A deep profiler’s guide to cytometry. Trends Immunol. 2012, 33(7):23-380

Wijkström H, Tribukait B. Deoxyribonucleic acid flow cytometry in predicting response to radical radiotherapy of bladder cancer. J Urol. 1990, 144(3):646-650

Krishan A, Ganapathi R. Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry. J Histochem Cytochem. 1979, 27(12):1655-1656

DeSouza N, Zhou M, Shan Y. Cell Cycle Analysis of CML Stem Cells Using Hoechst 33342 and Propidium Iodide. Methods Mol Biol. 2016, 1465:47-57

Oli M, Aptamer conjugated gold nanorods for targeted nanothermal radiation of Glioblastoma cancer cells. A novel selective targeted approach to cancer treatment. Young Sci J. 2010, 3:18-25

Srinivas O, Larrieu SP, Duverger E, Boccaccio S, Bousser MT, Monsigny M, Fonteneau JF, Jotereau F, Roche AC, Synthesis of Glycocluster-Tumor Antigenic Peptide Conjugates for Dendritic Cell Targeting. Bioconjugate Chem. 2007, 18:1547-1554

Zhang P, Chiu YC, Tostanoski LH, Jewell CM. Polyelectrolyte Multilayers Assembled Entirely from Immune Signals on Gold Nanoparticle Templates Promote Antigen-Specific T Cell Response. ACS Nano. 2015, 9(6): 6465-6477

Baganz NL, Blakely RD. A Dialogue between the Immune System and Brain, Spoken in the Language of Serotonin. ACS Chem Neurosci. 2013, 4:48-63

Antognoni P, Terry NHA, Richetti A, Luraghi R, Tordiglione M, Danova M. The predictive role of flow cytometry-derived tumor potential doubling time (Tpot) in radiotherapy: open questions and future perspectives. Int J Oncol. 1998, 12(2):245-256

Chang Q, Hedley D. Emerging applications of flow cytometry in solid tumor biology. Methods. 2012, 57:359-36


Refbacks

  • There are currently no refbacks.


AJCR(ISSN 2572-5769) Copyright © 2012-2021. All rights reserved. Published by Ivy Union Publishing, 3204 Valley Rush Dr, Apex, North Carolina 27502, United States