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Study of the Altered Anisotropy of Erythrocyte Ghost Membrane upon Interaction with Phytoreduced Negatively Charged Gold Nanoparticle from Celosia cristata and Vigna radiata

Monalisa Chakraborty, Sukhen Das, Ruma Basu, Papiya Nandy


Synthesis of negatively charged gold nanoparticles (GNP) was done from seed extract and seed electrolyte of two locally available plant sources Celosia cristata and  Vigna radiata without using any toxic chemicals and it is a rapid reduction way. Standard techniques of spectroscopy and   advanced  microscopic study was done in characterizing the particles and simultaneously the phytochemicals present in the electrolyte and extract were also spotted using established process. The effects of nanoparticles on live red blood cells were analyzed by calculating hemolysis percentage. Evaluations of GNP activity when interacted with erythrocyte ghost membrane and their impact on membrane fluidity and anisotropy were also studied as negatively charged GNP are less delineated in previous history.

 The results showed that negatively charged gold nanoparticles were reduced by flavonoids and phenols present in seed extract and electrolyte of plants and they are hemocompatible and can help to fluidize the membrane of erythrocyte ghost cells.

The whole experiment suggested a simple, ecofriendly approach of synthesizing negatively charged nanogold which are safe to live cells that can change the fluidity parameter of membrane constituent by decreasing the anisotropy which can further help in curing health problem related with cell membrane issue.


Green synthesis; gold nanoparticle; anisotropy; Phytochemicals; erythrocyte cell; fluidity.

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