Cover Image

Study of the Altered Anisotropy of Erythrocyte Ghost Membrane upon Interaction with Phytoreduced Negatively Charged Gold Nanoparticle from Celosia cristata and Vigna radiata

Monalisa Chakraborty, Sukhen Das, Ruma Basu, Papiya Nandy

Abstract


Synthesis of negatively charged gold nanoparticles (GNP) was done from seed extract and seed electrolyte of two locally available plant sources Celosia cristata and  Vigna radiata without using any toxic chemicals and it is a rapid reduction way. Standard techniques of spectroscopy and   advanced  microscopic study was done in characterizing the particles and simultaneously the phytochemicals present in the electrolyte and extract were also spotted using established process. The effects of nanoparticles on live red blood cells were analyzed by calculating hemolysis percentage. Evaluations of GNP activity when interacted with erythrocyte ghost membrane and their impact on membrane fluidity and anisotropy were also studied as negatively charged GNP are less delineated in previous history.

 The results showed that negatively charged gold nanoparticles were reduced by flavonoids and phenols present in seed extract and electrolyte of plants and they are hemocompatible and can help to fluidize the membrane of erythrocyte ghost cells.

The whole experiment suggested a simple, ecofriendly approach of synthesizing negatively charged nanogold which are safe to live cells that can change the fluidity parameter of membrane constituent by decreasing the anisotropy which can further help in curing health problem related with cell membrane issue.


Keywords


Green synthesis; gold nanoparticle; anisotropy; Phytochemicals; erythrocyte cell; fluidity.

Full Text:

PDF

References


Gangwar R, Dhumale V, Gosavi S, Sharma R and Datar S. Catalytic activity of allamanda mediated phytosynthesized anisotropic gold nanoparticles. Adv nat sci: Nanosci and Nanotechnol. 2013, 4:045005

Geddes CD, Porfenov A. Gryczynski L, Lakowicz JR. Luminescence blinking of gold nanoparticle. Chem Phys Lett. 2003, 380,269-272

Synthesis of Gold Nanoparticle http://www.researchgate.net/pubictopic.publicpostfileloader.html?id=5369883bd4c11850538b5259 (20may 2015)

Zhao L, Jianq D, Cai Y, Ji X, Xie R, Yang W. Tuning the size of gold nanoparticle in the citrate reduction by chloride ions.Nanoscale. 2012, 4:5071-5076

Pandey S, Oza G, Mewada A, Sharon M. Green synthesis of highly stable gold nanoparticle using momordica chanantia as nano fabricator. Arch Appl Sc Res. 2012, 4(2):1135-1141

Dongyan T, Dong Y, Ren H, Li L, He C. A review of phytochemistry, metabolite change and medicinal use of the common food mung bean and its sprouts (Vigna radiata) Chem Cent J. 2014, 8(4)

Makroo NR, Raina V, Bhatia A, Gupta R, Majid AR, Thakur KU, Rosamma LN. Evaluation of the red cell hemolysis in packed red cells during processing and storage Asian J Transfuse Sci. 2011, 5(1):15-17

The free dictionary about red cell ghost by Farlex. http://www.medical-dictionary.the free dictionary.com/red+cell+ghost.(Accessed on 21.04.2015)

Hoffman FJ. Physiological characteristics of Human Red Blood Cell Ghost J Gen Physicol. 42(1) (1958), 9-28

Ghosh S, Chakraborty MM, Das S, Basu R, Nandy P. Effect of different potencies of nanoparticle cuprum metallicum on membrane fluidity –A biophysical approach. Am J Homeo Med. 2014, 107(4) :161-169.

Print edition of the molecular probes hand book other nonpolar and amphiphlic probe.Probe for lipid and membrane. http://www.lifetechnologies.com/in/en/home/reference/molecularprobe-handbook/probe-forlipidsandmembrane/othernonpolarandamphiphilicprob

Chakraborty M, Dey A, Bala N, Das S, Basu R, Nandy P. Rapid single step green synthesis of copper oxide nanoparticle from Vigna radiata using three copper salts and study its antimicrobial nature. Int J Pharm. 2015, 5(1):93-97

Lu S, Duffin R, Poland C, Daly P, Murphy F, Drost E, et al. Efficacy of simple short term in vitro assay for predicting the potential of metal oxide nanoparticle to cause pulmonary inflammation Envirn Health Perspective. 2009, 117(2):241-247

Thompson P. Platelet and erythrocyte membrane fluidity changes in alcohol-dependent patient under acute withdrawal . Alcohol and Alcoholism. 1999, 34(3):49-354

Grebowski J, Krokosz A, Puchala M. Membrane fluidity and activity of membrane ATPase in human erythrocyte under influence of polyhydroxylated fullerene . Biochim Biophys Acta. 2013, 1828:241-248

Kassim M, Achoui M, Mustafa RM, Mohd AM, Yusoff KM. Ellagic acid,Phenolic acid and flavonoid in Malaysia honey extract demonstrate in vitro ant inflammatory activity. Nutr Res. 2010, 30(9) :650-659

Lin ZL, Harnly JM. A screening method for the identification of glycosylated flavonoid and other phenolic compounds using a standard analytical approach for all plant materials. J Agric Food Chem. 2007, 55(4):1084-1096

Coat J. Interpretation of infrared spectra –a practical approach.-Encyclopedia of analytical chemistry R.A Meyer Ed.Jhon wiley and sons ltd, Chichester. 2000:10815-10837

Spectroscopic characterization of nanoparticles for potential drug discovery.C. Mark ,Talbutt Ph.D. http://www2-shimadzu.com/application/ftir,uv-vis/ftiruv-1402pdf

Gopinath K, Arumugam A. Extracellular mycosynthesis of gold nanoparticle using Fusarium solani Appl Nanosci. 2014, 4:657-662

Fischer D, Li Y Ahlemeyer B, Krieglstein J, Kissel T. In vitro cytooxicity testing of polycations influence of polymer structure on cell viability and hemolysis. Biomat. 2003, 24(7):1121-11

Verma A, Stellacci FA. Effect of surface properties on nanoparticle-cell interaction s. Small. 2010, 6(1):12-21

Pytel E, Banaszczyk OM, Michalak KM, Broncel M. Increased oxidative stress and decreased membrane fluidity in erythrocytes of CAD patients. Biochemistry and cell biology. 2013, 9(5): 315-318


Refbacks

  • There are currently no refbacks.


AJNNR ISSN 2574-6251)Copyright © 2012-2020. All rights reserved. Published by Ivy Union Publishing, 3204 Valley Rush Dr, Apex, North Carolina 27502, United States